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Human U2OS Cells – profiling bioactive compounds using Cell Painting

Accession number BBBC036 · Version 1 Example images Description of the biological application Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This...

BBBC999

Accession number [BBBCDATASETLINK] · Version [_] Example images Biological application Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nulla pharetra nibh eu massa sodales finibus. Nullam vulputate, quam at finibus lobortis, metus ex efficitur nulla, eu interdum libero massa eu erat. Sed...

Cell Cycle Jurkat Cells

Accession number BBBC048 · Version 1 Example images Biological application Abstract: We show that deep convolutional neural networks combined with nonlinear dimension reduction enable reconstructing biological processes based on raw image data. We demonstrate this by reconstructing the cell cycle of...

Broad Bioimage Benchmark Collection

The Broad Bioimage Benchmark Collection (BBBC) is a collection of freely downloadable microscopy image sets. In addition to the images themselves, each set includes a description of the biological application and some type of "ground truth" (expected results).

Benchmarking Methodology

To reasonably compare, or benchmark, algorithms for image analysis, researchers must agree on a common reference point—the image set's "correct" answer—and see how closely each algorithm matches it. We adopt the common term ground truth for this reference point even though it may be questionable in...

Drosophila Kc167 cells

Accession number BBBC002 · Version 1 Example image Biological application Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and...

Human U2OS cells (out of focus)

Accession number BBBC006 · Version 1 Example images Ground truth: in focus Out of focus Ground truth: foreground Biological application Since robust foreground/background separation and segmentation of cellular objects (i.e., identification of which pixels below to which objects) strongly depends on...

Collective cell migration

Accession number BBBC019 · Version 2 Example images DIC image Ground truth DIC image Ground truth Biological application The wound-healing assay is considered the gold standard for studying cell motility and migration. The assay is performed by time-lapse imaging (usually by phase contrast, or...