C. elegans live/dead assay

Accession number BBBC010 · Version 1

Example images

 mostly alive  mostly dead
Positive: mostly alive Negative: mostly dead


Biological application

This selection of images are controls selected from a screen to find novel anti-infectives using the roundworm C.elegans . The animals were exposed to the pathogen Enterococcus faecalis and either untreated or treated with ampicillin, a known antibiotic against the pathogen. The untreated (negative control) worms display predominantly the "dead" pheotype: worms appear rod-like in shape and slightly uneven in texture. The treated (ampicillin, positive control) worms display predominantly the "live" phenotype: worms appear curved in shape and smooth in texture.

For more information, please see Moy et al. (ACS Chem Biol, 2009).


One image per channel (Channel 1 = brightfield; channel 2 = GFP) was acquired at MGH on a Discovery-1 automated microscope (Molecular Devices). Original image size is 696 x 520 pixels. Images are available in 16-bit TIF.

 BBBC010_v2_images.zip (70 MB)

Ground truth Biological labels button BForeground background button FOutlines button O

Biological labels button B

The 100 images are from a 384-well plate of positive and negative controls. The images are named using this format: <plate>_<wellrow>_<wellcolumn>_<wavelength>_<fileid>.tif Columns 1-12 are positive controls treated with ampicillin. Columns 13-24 are untreated negative controls.

Foreground background button F

We also provide human-corrected binary images of foreground/background segmentation:

 BBBC010_v1_foreground.zip (1.1 MB)

Outlines button O

To address the problem of correctly segmenting individual worms also when they overlap or cluster, we provide one binary foreground/background segmentation ground truth image for each worm:

 BBBC010_v1_foreground_eachworm.zip (2.7 MB)

For more information

These images were originally acquired for a screen in Fred Ausubel's lab at MGH. Please contact aconery AT molbio.mgh.harvard.edu for more information.

Published results using this image set

Outlines button O With automatically identified foreground Outlines button O With manually corrected foregrund Citation
81% 94% Wählby et al., Nat Meth, 2012

Recommended citation

"We used the C.elegans infection live/dead image set version 1 provided by Fred Ausubel and available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]."