Accession number BBBC012 · Version 1

Example images

Positive: pmk-1(km25)	Negative: wild type

Biological application

This selection of images show transgenic C. elegans expressing the promoter of gene clec-60 fused to GFP. The clec-60 gene is up-regulated during S. aureus infection [Irazoqui et al. PLoS Path 2010]. GFP expression is constrained to the posterior intestinal cells of wild type worms (wt, negative control), while the mutant (positive control), pmk-1(km25) is defective in the p38 MAPK homolog PMK-1 and often has elevated expression of clec-60p:GFP in the anterior intestine close to the pharynx, which is labeled with the red fluorescent mCherry.

Images

The images were acquired at MGH on using a Discovery-1 microscope (Molecular Devices), with one image per channel (Channel 1 = brightfield, Channel 2 = GFP, Channel 3 = mCheery). Original image size is 696 x 520 pixels. Images are available in 16-bit TIF.

 BBBC012_v1_images.zip (117 MB)

Ground truth 

The images provided are 1 replicate (48 images) of each of the two controls (positive, pmk-1, and negative, wt). Images of the pmk-1 mutant images are in the folder entitled "pmk_plate2" and wt worms are in the folder entitled "WT_plate2".

For more information

These images were originally aquired by Javier Irazoqui's lab at MGH. Please contact javier 'at' molbio.mgh.harvard.edu for more information.

Published results using this image set

Recommended citation

"We used image set BBBC012v1 provided by Javier Irazoqui, available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]."