Human U2OS cells transfluor

Accession number BBBC015 · Version 1

Example images

Positive Negative



Biological application

The images are of a human osteosarcoma cell line (U2OS) co-expressing beta2 (b2AR) adrenergic receptor and arrestin-GFP protein molecules. The receptor was modified-type that generates "vesicle-type" spots upon ligand stimulation.


The plate was acquired on iCyte imaging cytometer with iCyte software version 2.5.1. Image file format is JPEG with one image for green channel and one image for crimson channel. Image size is 1000 x 768 pixels. (23 MB)

Ground truth  Biological labels button B

This image set has a portion of a 96-well plate containing 3 replica rows and 12 concentration points of isoproterenol. In each well four fields were acquired. File name structure: __.JPG

BBBC015_v1_platemap.xls (17 kB)

The following file encodes this plate map in the format expected by CellProfiler's LoadText module:

BBBC015_v1_platemap.txt (859 b)

Published results using this image set

Z'-factor  V-factor  Citation
0.69*   Carpenter et al., Genome Biol., 2006
  0.63* Logan and Carpenter, J. Biomol. Screening, 2010


* Texture of speckles in green channel

For files to reproduce results in Logan and Carpenter, J. Biomol. Screening, 2010, see image set BBBC013.

For more information

These images were originally gathered for Ilya Ravkin's Invitation to Participate in the Comparison of Image Analysis Algorithms for Intracellular Screening.

Recommended citation

"We used image set BBBC015v1 provided by Ilya Ravkin, available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]."


Creative Commons LicenseThe BBBC015 images are licensed under a Creative Commons Attribution 3.0 Unported License by Ilya Ravkin.