Polymerized structures

Accession number BBBC028 · Version 1

Example images

DIC Ground Truth
DIC image Ground truth


This dataset includes 60 Differential Interference Contrast (DIC) images of 6 different shapes, such as triangle, circle and multi-height objects. The shapes were made by two-photon polymerization technique. The images are taken on an Olympus Cell-R microscope with a 20x lens.


Ground Truth 

The objects are created from SU-8 photoresist with rhodamine 6G, thus they are visible under fluorescent microscopes with the appropriate excitation light and filter. We include fluorescent images of the structures after switching from DIC mode and without moving the stage. The fluorescent images can be used for quality control measurements of DIC reconstruction algorithms. The suggested metric for the measurement is the Mean Squared Error (MSE) between the corresponding reconstructed and fluorescent images.


Published results using this image set

The proposed data set will be evaluated in a publication to be submitted.

Recommended citation

"We used image set BBBC028v1 [Koos, K., Molnár, J., Kelemen, L., Tamás, G., & Horvath, P. (2016). DIC image reconstruction using an energy minimization framework to visualize optical path length distribution. Scientific reports, 6.] from the Broad Bioimage Benchmark Collection."


Creative Commons LicenseThe BBBC028v1 image set are licensed under a Creative Commons Attribution 3.0 Unported License by Peter Horvath and Krisztián Koós.