Accession number BBBC016 · Version 1
Description of the biological application
This image set is of a Transfluor assay where an orphan GPCR is stably integrated into the b-arrestin GFP expressing U2OS cell line. After one hour incubation with a compound the cells were fixed with (formaldehyde).
The plate was read on Cellomics ArrayScan HCS Reader using the GPCR Bioapplication. File format is 8-bit TIFF with one image for green channel (GFP) and one image for blue channel (DNA). Image size is 512 x 512 pixels.
The images constitute one row of a 384 well plate. The dose curve consists of 11 dose points and one control. Each concentration is duplicated in adjacent wells. Each well has three fields. File name structure: _.TIF
The following file encodes this plate map in the format expected by CellProfiler's LoadText module:
Published results using this image set
|0.43*||Carpenter et al., Genome Biol., 2006|
|0.67†||Logan and Carpenter, J. Biomol. Screening, 2010|
* Integrated intensity on edge of speckles in green channel
† Radial distribution CV of intensity
Files to reproduce results in Logan and Carpenter, J. Biomol. Screening, 2010 (includes CellProfiler pipelines and metadata):
For more information
These images were originally gathered for Ilya Ravkin's Invitation to Participate in the Comparison of Image Analysis Algorithms for Intracellular Screening.