This image set is of a Transfluor assay where an orphan GPCR is stably integrated into the b-arrestin GFP expressing U2OS cell line. After one hour incubation with a compound the cells were fixed with (formaldehyde).
Images
The plate was read on Cellomics ArrayScan HCS Reader using the GPCR Bioapplication. File format is 8-bit TIFF with one image for green channel (GFP) and one image for blue channel (DNA). Image size is 512 x 512 pixels.
The images constitute one row of a 384 well plate. The dose curve consists of 11 dose points and one control. Each concentration is duplicated in adjacent wells. Each well has three fields. File name structure: _.TIF