LPS activated immortalized mouse migroglia (IMG)


Accession number BBBC054 · Version 1

Example images

Biological application

Each field of view correspond to an exposure time of LPS with 30 minute intervals between 0 hours and 30 hours. At different exposure times we expect the proportions of cell phenotypes to change, indicating the progression of the immune response to LPS.


Images were collected using a Leica DMI 6000 microscope with a 20x objective, through an Andor (AND-DG-152V-C1E-FI) camera, using Metamorph Premiere Software. The images are in TIFF format.

Replicate 1

Replicate 1 Annotation

Replicate 2

Replicate 3

Ground truth Biological labels button B

A CSV with four columns: index, axis-0, axis-1, axis-2, and label. Each row in the CSV corresponds to a location of a cell (not necessarily centroid). 'index' indicates the annotation number. 'axis-0' indicates which FOV/image the coordinate is in. 'axis-1' indicates the x-coordinate, and 'axis-2' indicates the y-coordinate (in pixels). The column 'label' indicates the manually annotated phenotype of the cell (one of 'round', 'amoeboid', and 'stratified').

For more information

This dataset was created at Caltech, BBE, Pasadena, CA. Please contact Valentine Svensson regarding this dataset.

Published results using this image set


Recommended citation

"We used image set [BBBC054], available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]." 


The images and ground truth are licensed under a Creative Commons Attribution 3.0 Unported License (Commercial use allowed)