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Contribute to the BBBC

We welcome contributions of the following: Image sets with descriptions and ground truth High-throughput image sets Published benchmarking results of algorithms applied to the BBBC image sets Corrections to existing descriptions or ground truth Feedback on the benchmarking methodology or other...

Human HT29 colon-cancer cells

Accession number BBBC008 · Version 1 Example images Biological application These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. A set of about 43,000 such images was used by Moffat et al. (Cell, 2006) to...

Murine bone-marrow derived macrophages

Accession number BBBC020 · Version 1 Note: BBBC has received a complaint that "BBB020_v1_outlines_nuclei" appears incomplete and we have been unable to obtain the missing images from the original contributor. Example Images Merged CD11b/APC DAPI Biological application These images depict bone-marrow...

Synthetic DIC Images

Accession number BBBC029 · Version 1 Example images DIC images Ground truth Images The dataset includes 218 simulated Differential Interference Contrast (DIC) images of 20 different binary and grayscale objects with various rotations. The images are available in noise-free version as well as with 20...

Mouse embryo blastocyst cells

Accession number BBBC032 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered not only in XY plane but also in XZ and YZ planes. Manually annotated ground truth provides a reference for image...

Induced pluripotent human stem cells

Accession number BBBC034 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered and have different intensity. This image set contains a clustered monolayer of nuclei that can be difficult to segment...

C. elegans live/dead assay

Accession number BBBC010· Version 1 Example images Positive: mostly alive Negative: mostly dead Biological application This selection of images are controls selected from a screen to find novel anti-infectives using the roundworm C.elegans . The animals were exposed to the pathogen Enterococcus...

Simulated 24-well plate with synthetic cells

Accession number BBBC031 · Version 1 Example images Description of the biological application We generated a synthetic HCS dataset simulating drugs perturbing the cell shape and the expressions of proteins (here represented by different colours). The dataset is useful for testing classification and...

Human U2OS cells – Small molecule Cell Painting experiment

Accession number BBBC040 · Version 1 Example images Biological application An ideal small-molecule screening library would be enriched in non-redundant bioactive compounds that maximize the performance diversity of the library. The construction of such libraries would be aided by a rapid feedback...

Nuclei of mouse embryonic cells

Accession number BBBC050 · Version 2 Example images Biological application Cell dynamics during the early mouse embryogenesis change spatiotemporally. For understanding the mechanism of this developmental process, imaging cell dynamics by live-cell imaging of fluorescently labeled nuclei and...