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Human U2OS Cells – profiling bioactive compounds using Cell Painting

Accession number BBBC036 · Version 1 Example images Description of the biological application Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This...

BBBC999

Accession number [BBBCDATASETLINK] · Version [_] Example images Biological application Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nulla pharetra nibh eu massa sodales finibus. Nullam vulputate, quam at finibus lobortis, metus ex efficitur nulla, eu interdum libero massa eu erat. Sed...

Cell Cycle Jurkat Cells

Accession number BBBC048 · Version 1 Example images Biological application Abstract: We show that deep convolutional neural networks combined with nonlinear dimension reduction enable reconstructing biological processes based on raw image data. We demonstrate this by reconstructing the cell cycle of...

Image Sets

Identification and segmentation Accession Description Mode Fields per Sample Total Fields Total Images Ground Truth BBBC001 Human HT29 colon-cancer cells Fluorescent 6 6 6 BBBC002 Drosophila Kc167 cells Fluorescent 5 50 50 BBBC003 Mouse embryos DIC 15 15 15 BBBC004 Synthetic cells Fluorescent 20 100...

Synthetic cells

Accession number BBBC005 · Version 1 Example images In focus Out of focus True foreground Biological application Since robust foreground/background separation and segmentation of cellular objects (i.e.,identification of which pixels below to which objects) strongly depends on image quality, focus...

Drosophila Kc167 cells

Accession number BBBC007 · Version 1 Example images DNA Actin Outlined nuclei Outlined cells Biological application Drosophila melanogaster Kc167 cells were stained for DNA (to label nuclei) and actin (a cytoskeletal protein, to show the cell body). Automatic cytometry requires that cells be...

Nuclei of U2OS cells in a chemical screen

Accession number BBBC039 · Version 1 Example images Description of the biological application This image set is part of a high-throughput chemical screen on U2OS cells, with examples of 200 bioactive compounds. The effect of the treatments was originally imaged using the Cell Painting assay...

C. elegans infection marker

Accession number BBBC012 · Version 1 Example images Positive: pmk-1(km25) Negative: wild type Biological application This selection of images show transgenic C. elegans expressing the promoter of gene clec-60 fused to GFP. The clec-60 gene is up-regulated during S. aureus infection [Irazoqui et al...

Human HT29 colon-cancer cells

Accession number BBBC001 · Version 1 Example image Biological application These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. For instance, Moffat et al. ( Cell, 2006) screened a set of about 43,000 such...

Synthetic cells

Accession number BBBC004· Version 1 Example images Image Foreground Biological application One of the principal challenges in counting or segmenting nuclei is dealing with clustered nuclei. To help assess algorithms' performance in this regard, this synthetic image set consists of five subsets with...