To reasonably compare, or benchmark, algorithms for image analysis, researchers must agree on a common reference point—the image set's "correct" answer—and see how closely each algorithm matches it. We adopt the common term ground truth for this reference point even though it may be questionable in...

Accession number BBBC002 · Version 1 Example image Biological application Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and...

Accession number BBBC006 · Version 1 Example images Ground truth: in focus Out of focus Ground truth: foreground Biological application Since robust foreground/background separation and segmentation of cellular objects (i.e., identification of which pixels below to which objects) strongly depends on...

Accession number BBBC019 · Version 2 Example images DIC image Ground truth DIC image Ground truth Biological application The wound-healing assay is considered the gold standard for studying cell motility and migration. The assay is performed by time-lapse imaging (usually by phase contrast, or...

Accession number BBBC024 · Version 1 Example images 3D image 3D foreground Biological application One of the principal challenges in counting or segmenting nuclei is dealing with clustered nuclei. To help assess algorithms' performance in this regard, this synthetic image set consists of four...

Accession number BBBC025 · Version 1 Morphological profiles of RNAi-induced gene knockdown are highly reproducible but dominated by seed effects Abstract: RNA interference and morphological profiling - the measurement of thousands of phenotypes from individual cells by microscopy and analysis image...

Accession number BBBC026 · Version 1 Biological application This 384-well plate has images of co-cultured hepatocytes and fibroblasts. Every other well is populated (A01, A03, ..., C01, C03, ...) such that 96 wells comprise the data. Each well has 9 sites and thus 9 images associated, totaling 864...

Accession number BBBC033 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered. Mouse trophoblast stem cells image contains a clustered monolayer of nuclei that can be difficult to segment. This...

Accession number BBBC035 · Version 1 Example images Description of the biological application These are synthetic images from the Cell Tracking Challenge. The images depict simulated nuclei of HL60 cells stained with Hoescht (training datasets). These synthetic images of HL60 cells provide an...

Accession number BBBC014 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the transcription factor NFκB in MCF7 (human breast adenocarcinoma cell line) and A549 (human alveolar basal epithelial) cells in...